Messi is yet to return from holiday following international duty at the Copa America, meaning he will not feature against Arsenal at the Nou Camp.
Argentina came third in the tournament but the 32-year-old has since been banned from international football for three months after claiming the competition was corrupt.
Messi was sent off in the 2-1 over Chile and subsequently said the “cup was fixed for Brazil”, triggering a suspension and a $50,000 fine.
The Barcelona talisman has been enjoying a well-earned holiday following the tournament but will be back with his club before the start of the La Liga campaign.
Meanwhile, Luis Suarez has made his return to training after a similar break following his Copa America exploits wth Uruguay.
The striker is expected to lead the line against Arsenal and could be supported by Ousmane Dembele and summer signing Antoine Griezmann.
It remains to be seen if Philippe Coutinho will play in the friendly after the Brazilian has been linked with a shock loan move to the Emirates.
Reports suggest talks between the two clubs will take place surrounding the fixture, meaning Coutinho may not be in a position to feature for the home side.
Frenkie de Jong could make his first start for Barcelona after being utilised off the bench in both the previous pre-season friendlies.
The Dutchman is expected to partner Sergio Busquets and Ivan Rakitic, although Carles Alena, Sergi Roberto and Rafinha can also play in the middle.
Gerard Pique is back in training, having missed the friendly against Vissel Kobe, and should partner Samuel Umtiti in central defence while Jordi Alba and Nelson Semedo could start in the full-back role.
Meanwhile, Malcom has travelled to Russia in order to complete a move to Zenit St Petersburg.
Barcelona predicted line-up vs Arsenal – Joan Gamper Trophy
(4-3-3): Ter Stegen; Semedo, Pique, Umtiti, Alba; Busquets, De Jong, Rakitic; Dembele, Griezmann, Suarez